Nuclear localizing sequences promote nuclear translocation and enhance the radiotoxicity of the anti-CD33 monoclonal antibody HuM195 labeled with 111In in human myeloid leukemia cells.

@article{Chen2006NuclearLS,
  title={Nuclear localizing sequences promote nuclear translocation and enhance the radiotoxicity of the anti-CD33 monoclonal antibody HuM195 labeled with 111In in human myeloid leukemia cells.},
  author={Paul Chen and Judy Wang and Kristin Lofthus Hope and Liqing Jin and John R Dick and Ross Cameron and Joseph E Brandwein and M. L. Minden and Raymond M Reilly},
  journal={Journal of nuclear medicine : official publication, Society of Nuclear Medicine},
  year={2006},
  volume={47 5},
  pages={827-36}
}
UNLABELLED Our objective was to evaluate the toxicity of the anti-CD33 monoclonal antibody HuM195 modified with peptides (CGYGPKKKRKVGG) harboring the nuclear localizing sequence (NLS; underlined) of simian virus 40 large T antigen and labeled with (111)In against acute myeloid leukemia (AML) cells. METHODS HuM195 was derivatized with sulfosuccinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (sulfo-SMCC) to introduce maleimide groups for reaction with NLS-peptides and then conjugated… CONTINUE READING

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