Nuclear localization is required for function of the essential clock protein FRQ.

@article{Luo1998NuclearLI,
  title={Nuclear localization is required for function of the essential clock protein FRQ.},
  author={Chenglong Luo and Jennifer J. Loros and Jay C. Dunlap},
  journal={The EMBO journal},
  year={1998},
  volume={17 5},
  pages={1228-35}
}
The frequency (frq) gene in Neurospora encodes central components of a circadian oscillator, a negative feedback loop involving frq mRNA and two forms of FRQ protein. Here we report that FRQ is a nuclear protein and nuclear localization is essential for its function. Deletion of the nuclear localization signal (NLS) renders FRQ unable to enter into the nucleus and abolishes overt circadian rhythmicity, while reinsertion of the NLS at a novel site near the N-terminus of FRQ restores its function… CONTINUE READING
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The use of lacZ gene fusions in Neurospora crassa

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Alternative initiation of transcription and timespecific phosphorylation yield multiple forms of essential clock protein FREQUENCY

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