Nuclear import of APOBEC3F-labeled HIV-1 preintegration complexes.

@article{Burdick2013NuclearIO,
  title={Nuclear import of APOBEC3F-labeled HIV-1 preintegration complexes.},
  author={Ryan C. Burdick and Wei-Shau Hu and Vinay K Pathak},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 49},
  pages={E4780-9}
}
Human cytidine deaminases APOBEC3F (A3F) and APOBEC3G (A3G) are host factors that incorporate into virions and restrict virus replication. We labeled HIV-1 particles with yellow fluorescent protein (YFP)-tagged APOBEC3 proteins and examined their association with preintegration complexes (PICs) in infected cells. Labeling of PICs with A3F-YFP, and to a lesser extent A3G-YFP, could be used to visualize PICs in the nuclei, which was dependent on nuclear pore protein Nup153 but not TNPO3. We show… CONTINUE READING