Nuclear estradiol receptors in some nontarget organs of rats


Some properties of macromolecules, specifically binding estradiol (E2), obtained from KCl extracts of nuclei of the uterus, kidneys, liver, testes, and prostate of male and female rats were investigated. These macromolecules from the uterus and liver were shown to be extractable in the largest amounts from chromatin by KCl in a concentration of 0.6 M. The ability of nuclear extracts of the uterus, kidneys, and liver to bind E2 specifically was completely abolished by treatment with pronase, but not with RNase or DNase, thus indicating the protein nature of these macromolecules. Only estrogens and not testosterone, 5α-dihydrotestosterone progesterone, or corticosterone, competed for these E2-binding sites of the macromolecules from nuclear extracts of all the organs tested.

DOI: 10.1007/BF00800176

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@article{Smirnov2004NuclearER, title={Nuclear estradiol receptors in some nontarget organs of rats}, author={Alexander N. Smirnov and O. V. Smirnova and U. M. Fisher and V. B. Rozen}, journal={Bulletin of Experimental Biology and Medicine}, year={2004}, volume={81}, pages={85-87} }