Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes

@article{Lnrt2003NuclearEB,
  title={Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes},
  author={P{\'e}ter L{\'e}n{\'a}rt and Gw{\'e}na{\"e}l Rabut and Nathalie Daigle and Arthur R Hand and Mark Terasaki and Jan Ellenberg},
  journal={The Journal of Cell Biology},
  year={2003},
  volume={160},
  pages={1055 - 1068}
}
Breakdown of the nuclear envelope (NE) was analyzed in live starfish oocytes using a size series of fluorescently labeled dextrans, membrane dyes, and GFP-tagged proteins of the nuclear pore complex (NPC) and the nuclear lamina. Permeabilization of the nucleus occurred in two sequential phases. In phase I the NE became increasingly permeable for molecules up to approximately 40 nm in diameter, concurrent with a loss of peripheral nuclear pore components over a time course of 10 min. The NE… CONTINUE READING

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