Nuclear dynamics, mitosis, and the cytoskeleton during the early stages of colony initiation in Neurospora crassa.

@article{Roca2010NuclearDM,
  title={Nuclear dynamics, mitosis, and the cytoskeleton during the early stages of colony initiation in Neurospora crassa.},
  author={Maria Gabriela Roca and Hsiao-Che Kuo and Alexander Lichius and Michael Freitag and Nick D Read},
  journal={Eukaryotic cell},
  year={2010},
  volume={9 8},
  pages={1171-83}
}
Neurospora crassa macroconidia form germ tubes that are involved in colony establishment and conidial anastomosis tubes (CATs) that fuse to form interconnected networks of conidial germlings. Nuclear and cytoskeletal behaviors were analyzed in macroconidia, germ tubes, and CATs in strains that expressed fluorescently labeled proteins. Heterokaryons formed by CAT fusion provided a rapid method for the imaging of multiple labeled fusion proteins and minimized the potential risk of overexpression… CONTINUE READING
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