Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay.

@article{Span1999NuclearCV,
  title={Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay.},
  author={Marcello Span{\^o} and E Cordelli and Giorgio Leter and Francesco Lombardo and Andrea Lenzi and Loredana Gandini},
  journal={Molecular human reproduction},
  year={1999},
  volume={5 1},
  pages={
          29-37
        }
}
The sperm chromatin structure assay (SCSA) is a flow cytometric (FCM) technique which exploits the metachromatic properties of Acridine Orange to monitor the susceptibility of sperm chromatin DNA to in-situ acid denaturation. SCSA was used to study the chromatin structure variations of human spermatozoa in semen, both before and after swim-up and after cryopreservation. Semen samples were provided by 19 healthy normozoospermic subjects attending pre-marriage checks. Each sample was divided into… CONTINUE READING

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