Nuclear actin and myosin I are required for RNA polymerase I transcription
@article{Philimonenko2004NuclearAA, title={Nuclear actin and myosin I are required for RNA polymerase I transcription}, author={Vlada V. Philimonenko and Jian Zhao and Sebastian Iben and Hana Dingov{\'a} and Katar{\'i}na Kysel{\'a} and Michael P. Kahle and H. Zentgraf and Wilma A. Hofmann and Primal de Lanerolle and Pavel Hoz{\'a}k and Ingrid Grummt}, journal={Nature Cell Biology}, year={2004}, volume={6}, pages={1165-1172} }
The presence of actin and nuclear myosin I (NMI) in the nucleus suggests a role for these motor proteins in nuclear functions. We have investigated the role of actin and nuclear myosin I (NMI) in the transcription of ribosomal RNA genes (rDNA). Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription. Microinjection of antibodies against actin or NMI, as well as short interfering RNA-mediated depletion of NMI, decreased Pol I transcription in vivo…
376 Citations
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References
SHOWING 1-10 OF 41 REFERENCES
Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II
- BiologyNature Cell Biology
- 2004
A fundamental role for actin in the initiation of transcription by RNA polymerase II is suggested after it was demonstrated that antibodies directed against β-actin, but not muscle actin, inhibited transcription in vivo and in vitro.
hRRN3 is essential in the SL1‐mediated recruitment of RNA Polymerase I to rRNA gene promoters
- BiologyThe EMBO journal
- 2001
It is concluded that hRRN3 functions to recruit initiation‐competent Pol I to rRNA gene promoters, which suggests a mechanism for growth control of Pol I transcription.
Purification, assay, and properties of RNA polymerase I and class I-specific transcription factors in mouse.
- BiologyMethods in enzymology
- 1996
Rrn3 Phosphorylation Is a Regulatory Checkpoint for Ribosome Biogenesis*
- Biology, ChemistryThe Journal of Biological Chemistry
- 2002
Results suggest that the phosphorylation state of Rrn3 regulates rDNA transcription by determining the steady-state concentration of the Rrn 3·RNA polymerase I complex within the nucleolus.
Factor C*, the specific initiation component of the mouse RNA polymerase I holoenzyme, is inactivated early in the transcription process
- BiologyMolecular and cellular biology
- 1994
When elongation is halted before this critical distance, the C* remains active and on that template complex, greatly extending the kinetics of transcription and generating manyfold more transcripts than would have been synthesized if elongation had proceeded past the critical distance where C* is inactivated.
Life on a planet of its own: regulation of RNA polymerase I transcription in the nucleolus.
- BiologyGenes & development
- 2003
This review restricts the background information to the minimal level that is required for understanding initiation complex formation at the rDNA promoter before proceeding to review the regulatory pathways that adapt cellular rRNA synthesis to cell metabolism and growth.
Evidence for the Presence of Myosin I in the Nucleus*
- BiologyThe Journal of Biological Chemistry
- 1997
It is proposed that the 120-kDa protein is a previously undescribed myosin I isoform that is an intranuclear actin-based molecular motor.