Nuclear Gene Sequences from a Late Pleistocene Sloth Coprolite

@article{Poinar2003NuclearGS,
  title={Nuclear Gene Sequences from a Late Pleistocene Sloth Coprolite},
  author={Hendrik N. Poinar and Melanie Kuch and Gregory McDonald and Paul S. Martin and Svante P{\"a}{\"a}bo},
  journal={Current Biology},
  year={2003},
  volume={13},
  pages={1150-1152}
}

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References

SHOWING 1-10 OF 19 REFERENCES

Molecular phylogeny of the extinct ground sloth Mylodon darwinii.

Phylogenetic analyses using homologous sequences from all extant edentate groups suggest that Mylodon darwinii was more closely related to the two- toed than the three-toed sloths and, thus, that an arboreal life-style has evolved at least twice among sloths.

A molecular phylogeny of two extinct sloths.

DNA was extracted and mitochondrial cytochrome b gene sequences were determined from representatives of two extinct groups of sloths, their two living relatives, anteaters and armadillos, to strengthen the view that the two families of living sloths adapted independently to an arboreal life-style.

Nuclear DNA sequences from late Pleistocene megafauna.

The nuclear sequences retrieved from the mammoths suggest that mammoths were more similar to Asian elephants than to African elephants and under some circumstances, nucleotide sequence differences between alleles found within one individual can be distinguished from DNA sequence variation caused by postmortem DNA damage.

Neanderthal DNA: Not just old but old and cold?

The thermal history of a range of Holocene and Pleistocene bones whose DNA quality has been tested is calculated and it is found that in only very few sites with Neanderthal remains is the preservation of DNA likely to match the quality of that from the skeleton found at Mezmaiskaya Cave.

Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis.

DNA from excrements can be amplified by means of the polymerase chain reaction. However, this has not been possible with ancient feces. Cross-links between reducing sugars and amino groups were shown

DNA extraction from Pleistocene bones by a silica-based purification method.

The following method, which is a modification of a protocol published by Boom et a/.

Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

  • S. Pääbo
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1989
The polymerase chain reaction can be used to amplify and study short mitochondrial DNA sequences that are of anthropological and evolutionary significance and opens up the prospect of performing diachronical studies of molecular evolutionary genetics.

DNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA.

It is shown that DNA molecules amplified by PCR from DNA extracted from animal bones and teeth that vary in age between 25 000 and over 50 000 years carry C-->T and G-->A substitutions, which are due to the occurrence of modified deoxycytidine residues in the template DNA.

The ear region of edentates and the phylogeny of the Tardigrada (Mammalia, Xenarthra)

A cladistic investigation of the phylogenetic relationships among 21 extinct and extant genera of sloths was performed on the basis of characteristics of the bony anatomy of the auditory region in order to evaluate specific hypotheses of relationship within the group.

Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus)

An assay designed to measure the amount of amplifiable nuclear DNA in low DNA concentration extracts from noninvasive samples is described and a new efficient approach for accurate microsatellite genotyping is formulated.