NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators

  title={NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators},
  author={Miguel A Vega-Palas and Enrique Flores and Antonia Herrero},
  journal={Molecular Microbiology},
The gene ntcA is required for full expression of proteins subject to ammonium repression in the cyanobacterium Synechococcus. A 3.1 kb DNA fragment able to complement an ntcA mutant was digested with exonuclease III, and deleted fragments of different sizes were tested for complementation of that mutant, allowing the localization of its mutation within a BamHI‐HindIII genomic fragment of c. 0.4 kb. Insertion of a chloramphenicol‐resistance‐encoding gene cassette into both the BamHI and the… 
Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC7120
Results showed that the ntcA gene product, NTCA, is required in Anabaena sp.
A Novel Gene (narM) Required for Expression of Nitrate Reductase Activity in the Cyanobacterium Synechococcus elongatus Strain PCC7942
Results suggested that narM is required for either synthesis of the prosthetic group of nitrate reductase or assembly of the prostate-based prosthetic groups to the NarB polypeptide to form functional nitrate reducesase in cyanobacteria.
Identification and characterization of two nitrogen-regulated genes of the cyanobacterium Synechococcus sp. strain PCC7942 required for maximum efficiency of nitrogen assimilation
Findings suggested that nirB is required for expression of maximum nitrite reductase activity, which seems to regulate utilization of fixed nitrogen by controlling the expression of a certain gene(s) involved in nitrogen metabolism.
Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development
The Anabaena sp. strain PCC 7120 ntcA (bifA) gene encodes a sequence-specific DNA-binding protein, NtcA (BifA, VF1) that interacts with the upstream region of several genes, including glnA, xisA,
Genes encoded on a cyanobacterial plasmid are transcriptionally regulated by sulfur availability and CysR
Results indicate that the accumulation of a large number of pANL transcripts is regulated by sulfur levels and CysR, and a strain in which the putative chromate resistance gene was interrupted by a drug resistance marker exhibited increased resistance to chromate when grown in media containing low sulfate concentrations.
Characterization of devH, a Gene Encoding a Putative DNA Binding Protein Required for Heterocyst Function inAnabaena sp. Strain PCC 7120
Nitrogen starvation of the devH mutant induces heterocysts of wild-type morphology, but the mutant is inviable in the absence of fixed nitrogen and unable to reduce acetylene aerobically.
Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120
A region of the genome of the filamentous, nitrogen-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 that contains a cluster of genes involved in nitrate assimilation has been
Transcription factor NtcB specifically controls the nitrate assimilation genes in the marine cyanobacterium Synechococcus sp. strain PCC 7002
Results demonstrate that NtcB specifically controls the transcription of the three genes for nitrate assimilation in this marine cyanobacterium.
Characterization of cis elements that regulate the expression of glnA in Synechococcus sp. strain PCC 7942
The results indicate that the glnA promoter is subject to complex regulation that involves sequences upstream and downstream from the transcription start site.
Regulated expression of glutamyl‐tRNA synthetase is directed by a mobile genetic element in the cyanobacterium Tolypothrix sp. PCC 7601
The genome of Tolypothrix sp. PCC 7601 carries two copies of a novel insertion sequence, ISTosp1. One of the two copies is located upstream of the gene encoding glutamyl‐tRNA synthetase, an enzyme


Role of the nac gene product in the nitrogen regulation of some NTR-regulated operons of Klebsiella aerogenes
A positive, genetic selection against the activity of the nitrogen regulatory (NTR) system was used to isolate insertion mutations affecting nitrogen regulation in Klebsiella aerogenes, showing that loss of nitrogen regulation resulted from the absence of nac function rather than from an altered form of the nac gene product.
Identification and molecular characterization of a transcriptional regulator from Pseudomonas aeruginosa PAO1 exhibiting structural and functional similarity to the FNR protein of Escherichia coli
  • R. Sawers
  • Biology, Engineering
    Molecular microbiology
  • 1991
The results imply that the mechanisms by which ANR and FNR regulate transcription are fundamentally similar.
Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942
Twenty-seven mutants that were unable to assimilate nitrate were isolated from Synechococcus sp. strain PCC 7942. In addition to mutants that lacked nitrate reductase or nitrite reductase, seven
Characterization and mutagenesis of sulfur-regulated genes in a cyanobacterium: evidence for function in sulfate transport.
Data suggest that a sulfate transport system similar to that of Synechococcus sp.
Anaerobic growth and cyanide synthesis of Pseudomonas aeruginosa depend on anr, a regulatory gene homologous with fnr of Escherichia coli
Pseudomonas fluorescens, Pseudomona putida, PseUDomonas syringae, and pseudomonas mendocina all had a functional analogue of ANR, indicating that similar anaerobic control mechanisms exist in these bacteria.
Molecular genetic analysis of FNR‐dependent promoters
By analysis of RNA produced in vivo the promoters of four FNR‐dependent genes were localized and shown to display a common arrangement, and a 22bp dyad symmetry was found about 30 nucleotides upstream of the transcriptional startpoints and a similar sequence was shown to overlap the site of transcription initiation in the negatively controlled fnr gene.
A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti
It is proposed that FixL senses oxygen through its haem moiety and transduces this signal by controlling the phos-phorylation of FixJ, and is an oxygen-binding haemoprotein.
Photosynthetic electron transport controls nitrogen assimilation in cyanobacteria by means of posttranslational modification of the glnB gene product.
It is proposed that the photosynthetic electron transport chain may regulate the pathway of nitrogen assimilation in cyanobacteria by means of posttranslational, covalent modification of the glnB gene product.
Actinobacillus pleuropneumoniae hlyX gene homology with the fnr gene of Escherichia coli
Comparison of the deduced amino acid sequence of the hlyX gene product (HlyX) with that of FNR revealed a high degree of well-aligned sequence correlation throughout the polypeptide chain, suggesting that HlyX may regulate, rather than mediate, hemolytic activity in E. coli.