We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.

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@inproceedings{Vinegoni2007NpgRJ_NMETH_11491, title={NpgRJ_NMETH_1149 1..3}, author={Claudio Vinegoni and Chrysoula Pitsouli and Daniel Razansky and Norbert Perrimon and Vasilis Ntziachristos}, year={2007} }