Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity.

@article{Dailey1990NovelYP,
  title={Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity.},
  author={David P. Dailey and Gary L. Schieven and Maggie Lim and Hans Marquardt and Thomas Gilmore and J. Thorner and G. Steven Martin},
  journal={Molecular and cellular biology},
  year={1990},
  volume={10 12},
  pages={6244-56}
}
Extracts of bakers' yeast (Saccharomyces cerevisiae) contain protein-tyrosine kinase activity that can be detected with a synthetic Glu-Tyr copolymer as substrate (G. Schieven, J. Thorner, and G.S. Martin, Science 231:390-393, 1986). By using this assay in conjunction with ion-exchange and affinity chromatography, a soluble tyrosine kinase activity was purified over 8,000-fold from yeast extracts. The purified activity did not utilize typical substrates for mammalian protein-tyrosine kinases… CONTINUE READING