Novel x-type high-molecular-weight glutenin genes from Aegilops tauschii and their implications on the wheat origin and evolution mechanism of Glu-D1-1 proteins.

@article{Zhang2008NovelXH,
  title={Novel x-type high-molecular-weight glutenin genes from Aegilops tauschii and their implications on the wheat origin and evolution mechanism of Glu-D1-1 proteins.},
  author={Yanzhen Z Zhang and Xiaohui Li and Aili Wang and Xueli An and Qian Zhang and Yuhe Pei and Liyan Gao and Wujun Ma and Rudi Appels and Yueming Yan},
  journal={Genetics},
  year={2008},
  volume={178 1},
  pages={23-33}
}
Two new x-type high-molecular-weight glutenin subunits with similar size to 1Dx5, designated 1Dx5*t and 1Dx5.1*t in Aegilops tauschii, were identified by SDS-PAGE, RP-HPLC, and MALDI-TOF-MS. The coding sequences were isolated by AS-PCR and the complete ORFs were obtained. Allele 1Dx5*t consists of 2481 bp encoding a mature protein of 827 residues with deduced Mr of 85,782 Da whereas 1Dx5.1*t comprises 2526 bp encoding 842 residues with Mr of 87,663 Da. The deduced Mr's of both genes were… CONTINUE READING

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