Novel versatile cryoprotectants for heavy-atom derivatization of protein crystals.

@article{Sugahara2006NovelVC,
  title={Novel versatile cryoprotectants for heavy-atom derivatization of protein crystals.},
  author={Michihiro Sugahara and Naoki Kunishima},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2006},
  volume={62 Pt 5},
  pages={
          520-6
        }
}
Determination of a suitable cryoprotectant agent condition is one of the time-consuming processes in protein X-ray crystallography. The adaptability of two novel versatile cryoprotectants (oil- and water-based types) has been evaluated by cryodiffraction experiments using crystals of five different proteins. The results suggest that complementary use of both oil- and water-based cryoprotectants enables the successful cryoprotection of protein crystals. Since the versatile cryoprotectants do not… CONTINUE READING