Novel tools for functional analysis of CD11c: activation-specific, activation-independent, and activating antibodies.

@article{Sadhu2008NovelTF,
  title={Novel tools for functional analysis of CD11c: activation-specific, activation-independent, and activating antibodies.},
  author={Chanchal Sadhu and Lee Hendrickson and Ken O Dick and Tamara G Potter and Donald E. Staunton},
  journal={Journal of immunoassay & immunochemistry},
  year={2008},
  volume={29 1},
  pages={42-57}
}
Functions and binding properties of four CD11c-specific mAbs are described here. The mAb 496B stimulated, while 496K inhibited ligand binding of CD11c. The stimulatory mAb, 496B, as well as the inhibitory mAbs BU15 and 496 K appear to act allosterically, as they do not bind the CD11c I domain. The mAb 3.9 bound preferentially to activated forms of CD11c and the binding was divalent cation dependent. CD11c binding to 3.9 recapitulates many of the integrin-ligand interactions. Our data suggest… CONTINUE READING