Novel technique for generating macrophage foam cells for in vitro reverse cholesterol transport studies[S]

Abstract

Generation of foam cells, an essential step for reverse cholesterol transport studies, uses the technique of receptor-dependent macrophage loading with radiolabeled acetylated LDL. In this study, we used the ability of a biologically relevant detergent molecule, lysophosphatidylcholine (lyso-PtdCho), to form mixed micelles with cholesterol or cholesteryl… (More)
DOI: 10.1194/jlr.M041327

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