Novel sulfated octa- and decasaccharides from squid cartilage chondroitin sulfate E: sequencing and application for determination of the epitope structure of the monoclonal antibody MO-225.

@article{Deepa2007NovelSO,
  title={Novel sulfated octa- and decasaccharides from squid cartilage chondroitin sulfate E: sequencing and application for determination of the epitope structure of the monoclonal antibody MO-225.},
  author={Sarama Sathyaseelan Deepa and Kittiwan Kalayanamitra and Yumi Ito and Prachya Kongtawelert and Shigeyuki Fukui and Shuhei Yamada and Tadahisa Mikami and Kazuyuki Sugahara},
  journal={Biochemistry},
  year={2007},
  volume={46 9},
  pages={2453-65}
}
A mixture of octa- and decasaccharides obtained by the digestion with the hyaluronidase of chondroitin sulfate E derived from squid cartilage was subfractionated into 20 and 23 different components, respectively, by anion-exchange HPLC. MALDI-TOF/MS was used to assign the sugar and sulfate composition of the putative octa- and decasaccharides, and a disaccharide composition analysis revealed the building blocks to be A- [GlcUAbeta1-3GalNAc(4S)], C- [GlcUAbeta1-3GalNAc(6S)], and E- [GlcUAbeta1… CONTINUE READING