Novel standards in the measurement of rat insulin granules combining electron microscopy, high-content image analysis and in silico modelling

@inproceedings{Fava2011NovelSI,
  title={Novel standards in the measurement of rat insulin granules combining electron microscopy, high-content image analysis and in silico modelling},
  author={Eugenio Fava and Jaber Dehghany and Joke Ouwendijk and Andreas Mueller and Antje Niederlein and Paul Verkade and Michael Meyer-Hermann and Michele Solimena},
  booktitle={Diabetologia},
  year={2011}
}
Knowledge of number, size and content of insulin secretory granules is pivotal for understanding the physiology of pancreatic beta cells. Here we re-evaluated key structural features of rat beta cells, including insulin granule size, number and distribution as well as cell size. Electron micrographs of rat beta cells fixed either chemically or by high-pressure freezing were compared using a high-content analysis approach. These data were used to develop three-dimensional in silico beta cell… CONTINUE READING