Novel serine penicillocarboxypeptidase CPD-S3 from Penicillium janthinellum IBT 3991: purification, characterization, and uses in peptide synthesis and modification.

@article{Dey1993NovelSP,
  title={Novel serine penicillocarboxypeptidase CPD-S3 from Penicillium janthinellum IBT 3991: purification, characterization, and uses in peptide synthesis and modification.},
  author={Estera Szwajcer Dey and Stig Aasmul-Olsen},
  journal={Enzyme and microbial technology},
  year={1993},
  volume={15 12},
  pages={
          1042-50
        }
}
A novel carboxypeptidase (CPD-S3) from Penicillium janthinellum IBT 3991 has been isolated in a two-step purification procedure by cation exchange and affinity chromatography. The enzyme is a serine carboxypeptidase with a denatured molecular mass determined by SDS of 62 kDa of which 32% is carbohydrate. The isoelectric point is 5.1. CPD-S3 exhibits a high stability towards organic solvents and elevated temperatures. Besides the carboxypeptidase activity, CPD-S3 exhibits esterase, amidase, and… CONTINUE READING
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