Novel reporter system to monitor early stages of the hepatitis B virus life cycle

  title={Novel reporter system to monitor early stages of the hepatitis B virus life cycle},
  author={Hironori Nishitsuji and Saneyuki Ujino and Yuko Shimizu and Keisuke Harada and Jing Zhang and M Sugiyama and Masashi Mizokami and Kunitada Shimotohno},
  booktitle={Cancer science},
A recombinant hepatitis B virus (HBV) expressing NanoLuc (NL) (HBV/NL) was produced by cotransfecting a plasmid containing a 1.2-fold HBV genome carrying the NL gene with a plasmid bearing a packaging-defective 1.2-fold HBV genome into a human hepatoma cell line, HepG2. We found that NL activity in HBV/NL-infected primary hepatocytes or sodium taurocholate cotransporting polypeptide-transduced human hepatocyte-derived cell lines increased linearly for several days after infection and was… CONTINUE READING
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