Novel real-time monitoring system for human cytomegalovirus-infected cells in vitro that uses a green fluorescent protein-PML-expressing cell line.

@article{Ueno2006NovelRM,
  title={Novel real-time monitoring system for human cytomegalovirus-infected cells in vitro that uses a green fluorescent protein-PML-expressing cell line.},
  author={Tomonori Ueno and Yoshito Eizuru and Harutaka Katano and T. Kurata and Tetsutaro Sata and Shinji Irie and Kiyoko Ogawa-Goto},
  journal={Antimicrobial agents and chemotherapy},
  year={2006},
  volume={50 8},
  pages={2806-13}
}
Promyelocytic leukemia (PML) bodies are discrete nuclear foci that are intimately associated with many DNA viruses. In human cytomegalovirus (HCMV) infection, the IE1 (for "immediate-early 1") protein has a marked effect on PML bodies via de-SUMOylation of PML protein. Here, we report a novel real-time monitoring system for HCMV-infected cells using a newly established cell line (SE/15) that stably expresses green fluorescent protein (GFP)-PML protein. In SE/15 cells, HCMV infection causes… CONTINUE READING

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