Novel proteinaceous infectious particles cause scrapie.

@article{Prusiner1982NovelPI,
  title={Novel proteinaceous infectious particles cause scrapie.},
  author={Stanley B. Prusiner},
  journal={Science},
  year={1982},
  volume={216 4542},
  pages={
          136-44
        }
}
  • S. Prusiner
  • Published 9 April 1982
  • Biology, Medicine
  • Science
After infection and a prolonged incubation period, the scrapie agent causes a degenerative disease of the central nervous system in sheep and goats. Six lines of evidence including sensitivity to proteases demonstrate that this agent contains a protein that is required for infectivity. Although the scrapie agent is irreversibly inactivated by alkali, five procedures with more specificity for modifying nucleic acids failed to cause inactivation. The agent shows heterogeneity with respect to size… Expand
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References

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Scrapie agent contains a hydrophobic protein.
TLDR
Employing preparations of the scrapie agent enriched 100- to 1000-fold with respect to protein, it was found that digestion by proteinase K destroyed more than 99.9% of the infectivity, providing good evidence for a protein and for hydrophobic domains within the scrapIE agent. Expand
Evidence for the low molecular weight nature of scrapie agent
TLDR
The data presented here describe a purification procedure resulting in scrapie infectivity which migrates during polyacrylamide gel electrophoresis as a defined area of infectivity in a region characteristic of low molecular weight nucleic acid. Expand
Evidence for an essential DNA component in the Scrapie agent
ONE of the enigmas of microbiology has been the nature of the aetiological agent causing scrapie disease in sheep and goats. The unusual resistance of infectivity to heat, formaldehyde, nucleases andExpand
Is the scrapie agent a virus?
TLDR
The present experiments were designed to determine if similar 14-nm virus-like particles could be isolated from hamster brains infected with scrapie agent and, if so, if the 14- nm virus- like particles induce scrapie in mice and hamsters. Expand
Reversible chemical modification of the scrapie agent.
TLDR
Chemical modification of the scrapie agent by diethyl pyrocarbonate reduced the titer 1000-fold and exposure of the inactivated agent to hydroxylamine resulted in complete restoration of infectivity. Expand
Inactivation of the Scrapie Agent by Near Monochromatic Ultraviolet Light
TLDR
Experiments with ionizing and ultraviolet radiations led Alper et al. to question whether the agent depended on replication of a nucleic acid moiety for proliferation, which was shown to occur also when the titre of the injected material had been reduced by irradiation in vitro4. Expand
Molecular properties, partial purification, and assay by incubation period measurements of the hamster scrapie agent.
TLDR
Measurements of incubation period length were related to the titer of the agent and the dilution of the inoculated sample, providing evidence in support of a protein component within the scrapie agent which is essential for maintenance of infectivity. Expand
Some Properties of the Scrapie Agent and Its Behavior in Mice
TLDR
The long incubation period, the slowly progressive course of the disease, and the degenerative changes in the central nervous system all are consistent with the view that the agent under study is the transmissible agent of scrapie. Expand
Attempts to release the scrapie agent from tissue debris.
TLDR
The results obtained suggest either that the scrapie agent is unstable except when bound to tissue debris or that it requires the presence of other brain components for the expression of its full activity. Expand
Scrapie: A transmissible and hereditary disease of sheep
TLDR
Recent work has shown that there are specific degenerative changes in the brain in the hypothalamic-hypophyseal system and in the olivo-ponto-cerebellar system and that scrapie is not naturally infectious. Expand
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