Novel procedure to investigate the effect of phosphorylation on protein complex formation in vitro and in cells.

Abstract

The identification of phosphorylation state-dependent interacting proteins provides clues as to the function of the phosphorylation. Techniques such as yeast two hybrid and co-immunoprecipitation do not employ a single species of fully phosphorylated proteins. This is a particular problem for substrates of glycogen synthase kinase-3 (GSK3), where multiple… (More)
DOI: 10.1021/bi702030w

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