Novel pre-mRNA splicing of intronically integrated HBV generates oncogenic chimera in hepatocellular carcinoma.

@article{Chiu2016NovelPS,
  title={Novel pre-mRNA splicing of intronically integrated HBV generates oncogenic chimera in hepatocellular carcinoma.},
  author={Yung-Tuen Chiu and John Kwong-Leong Wong and Shing-Wan Choi and Karen Man Fong Sze and Daniel W H Ho and Lo-Kong Chan and Joyce Man Fong Lee and Kwan Man and Stacey Shawn Cherny and Wan-ling Yang and Chun-Ming Wong and Pak C. Sham and Irene Oi-lin Ng},
  journal={Journal of hepatology},
  year={2016},
  volume={64 6},
  pages={
          1256-64
        }
}
BACKGROUND & AIMS Hepatitis B virus (HBV) integration is common in HBV-associated hepatocellular carcinoma (HCC) and may play an important pathogenic role through the production of chimeric HBV-human transcripts. We aimed to screen the transcriptome for HBV integrations in HCCs. METHODS Transcriptome sequencing was performed on paired HBV-associated HCCs and corresponding non-tumorous liver tissues to identify viral-human chimeric sites. Validation was further performed in an expanded cohort… CONTINUE READING