Novel post-replicative DNA modification in Streptomyces: analysis of the preferred modification site of plasmid pIJ101.

@article{Dyson1998NovelPD,
  title={Novel post-replicative DNA modification in Streptomyces: analysis of the preferred modification site of plasmid pIJ101.},
  author={Paul J Dyson and Michael W. Evans},
  journal={Nucleic acids research},
  year={1998},
  volume={26 5},
  pages={1248-53}
}
Both Streptomyces lividans and Streptomyces avermitilis have the ability to site specifically modify their DNA, rendering it susceptible to in vitro Tris-dependent double-strand cleavage. We have cloned a 160 bp fragment containing the preferred modification site of plasmid pIJ101 and, employing an in vitro primer extension assay, determined that the modifications occur at guanine residues on either strand separated by 3 bp. These guanines are located within a 6 bp palindromic 'core' sequence… CONTINUE READING

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