Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships.

@article{Beloqui2006NovelPO,
  title={Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships.},
  author={Ana Beloqui and Marcos Pita and Julio Polaina and Arturo Gengler Martinez-Arias and Olga V. Golyshina and Miren Zum{\'a}rraga and Michail M Yakimov and Humberto Garc{\'i}a-Arellano and Miguel Alcalde and Victor Manuel Fernandez and Kieran M. Elborough and Jos{\'e} M Andreu and Antonio Ballesteros and Francisco J Plou and Kenneth N. Timmis and Manuel Ferrer and Peter N Golyshin},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 32},
  pages={22933-42}
}
RL5, a gene coding for a novel polyphenol oxidase, was identified through activity screening of a metagenome expression library from bovine rumen microflora. Characterization of the recombinant protein produced in Escherichia coli revealed a multipotent capacity to oxidize a wide range of substrates (syringaldazine > 2,6-dimethoxyphenol > veratryl alcohol > guaiacol > tetramethylbenzidine > 4-methoxybenzyl alcohol > 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) >> phenol red… CONTINUE READING
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