Novel non-viral method for transfection of primary leukemia cells and cell lines

@article{Schakowski2004NovelNM,
  title={Novel non-viral method for transfection of primary leukemia cells and cell lines},
  author={Frank Schakowski and Peter Buttgereit and Martin Mazur and Angela M{\"a}rten and Bjoern Schoettker and Marcus Gorschlueter and Ingo G. H. Schmidt-Wolf},
  journal={Genetic Vaccines and Therapy},
  year={2004},
  volume={2},
  pages={1 - 1}
}
BACKGROUND: Tumor cells such as leukemia and lymphoma cells are possible targets for gene therapy. However, previously leukemia and lymphoma cells have been demonstrated to be resistant to most of non-viral gene transfer methods. METHODS: The aim of this study was to analyze various methods for transfection of primary leukemia cells and leukemia cell lines and to improve the efficiency of gene delivery. Here, we evaluated a novel electroporation based technique called nucleofection. This novel… CONTINUE READING

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