Novel nikkomycin analogues generated by mutasynthesis in Streptomyces ansochromogenes

@article{Feng2014NovelNA,
  title={Novel nikkomycin analogues generated by mutasynthesis in Streptomyces ansochromogenes},
  author={Chi Feng and Hongbo Ling and Deyao Du and Jihui Zhang and Guoqing Niu and Huarong Tan},
  journal={Microbial Cell Factories},
  year={2014},
  volume={13},
  pages={59 - 59}
}
BackgroundNikkomycins are competitive inhibitors of chitin synthase and inhibit the growth of filamentous fungi, insects, acarids and yeasts. The gene cluster responsible for biosynthesis of nikkomycins has been cloned and the biosynthetic pathway was elucidated at the genetic, enzymatic and regulatory levels.ResultsStreptomyces ansochromogenes ΔsanL was constructed by homologous recombination and the mutant strain was fed with benzoic acid, 4-hydroxybenzoic acid, nicotinic acid and… Expand
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TLDR
The previously described Streptomyces tendae nikC::aph mutant was used to mutasynthesize nikkomycins Bx and Bz, which contain 2-amino-4-hydroxy-3-methyl-4-(4'-hydroxyphenyl)butanoic acid as the peptidyl side chain. Expand
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TLDR
Two novel nikkomycins Lx and Lz are synthesized as main components by a genetically engineered mutant of S. tendae Tu901, isolated from mutants and as minor components from the culture filtrate of the wild type strain. Expand
Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production
TLDR
A high nikkomycins producing strain was obtained by introduction of an extra nikomycin biosynthetic gene cluster into the genome of S. ansochromogenes by conjugal transfer. Expand
New, Highly Active Nonbenzoquinone Geldanamycin Derivatives by Using Mutasynthesis
TLDR
The successful application of the mutasynthesis methodology for the generation of ansamitocin P-3 derivatives and reblastatin 2, which shows lower cytotoxicity than geldanamycin but has a higher affinity for Hsp90, is reported. Expand
The Streptomyces tendae Tü901 L-lysine 2-aminotransferase catalyzes the initial reaction in nikkomycin D biosynthesis.
TLDR
The results identified the nikC-encoded L-lysine 2-aminotransferase as the nikkomycin biosynthetic enzyme that catalyzes the initial reaction in nikomycin D biosynthesis. Expand
Nikkomycin biosynthesis: formation of a 4-electron oxidation product during turnover of NikD with its physiological substrate.
TLDR
A nikD-like reaction is implicated in the biosynthesis of an L-lysine-derived pyridyl moiety found in streptogramin group B antibiotics that are used as part of a last resort treatment for severe infections due to gram positive bacteria. Expand
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TLDR
It is proposed that NikK catalyses the introduction of the amino group into the ketohexuronic acid precursor of nikkomycins, indicating that an alkaline environment favours the deamination reaction. Expand
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
TLDR
It is confirmed that SanH is essential for the hydroxylation of pyridyl residue in nikkomycin biosynthesis of S. ansochromogenes and first demonstrated that SanI is an effective electron donor for SanH, but not for SanQ in vivo. Expand
Formation of β-Hydroxy Histidine in the Biosynthesis of Nikkomycin Antibiotics
TLDR
The results reported here substantiate the earlier proposal that the covalent tethering of an amino acid onto a carrier protein domain prior to downstream modification is a general strategy for diverting a fraction of the amino acid into secondary metabolism. Expand
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TLDR
The results reported here substantiate the earlier proposal that the covalent tethering of an amino acid onto a carrier protein domain prior to downstream modification is a general strategy for diverting a fraction of the amino acid into secondary metabolism. Expand
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