Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices

@article{Wagner2017NovelMF,
  title={Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices},
  author={Jeff Wagner and Zhong-Min Wang and Sutapa Ghosal and Chelsea M. Rochman and Margy Gassel and Stephen Wall},
  journal={Analytical Methods},
  year={2017},
  volume={9},
  pages={1479-1490}
}
This work presents alternative extraction and analysis techniques to identify microplastics in the environment. This study aims to address previously noted issues with methods that use aggressive extraction treatments or optical microscopy identification techniques alone. Pulsed ultrasonic extraction with ultrapure water was used to remove microplastics from fish stomachs without dissolving the stomach tissues or microplastics. The technique is relatively simple and minimizes issues with… 

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References

SHOWING 1-10 OF 20 REFERENCES
Methodology Used for the Detection and Identification of Microplastics—A Critical Appraisal
Microplastics in aquatic ecosystems and especially in the marine environment represent a pollution of increasing scientific and societal concern, thus, recently a substantial number of studies on
Isolation of microplastics in biota-rich seawater samples and marine organisms
TLDR
The efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biological material from marine surface trawls to reveal any microplastics present is investigated and it is illustrated that enzyme digestion can aid the detection of microplastic debris within seawater samples and marine biota.
Microplastic pollution in the surface waters of the Laurentian Great Lakes.
...
1
2
...