Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori.

Abstract

A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori. Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method was rapid and reproducible, and it reduced PCR product contamination risk.

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@article{Gibson1999NovelMF, title={Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori.}, author={J R Gibson and Nick Saunders and Bryce Burke and R. J. Owen}, journal={Journal of clinical microbiology}, year={1999}, volume={37 11}, pages={3746-8} }