Novel method for identifying bacterial mutants with reduced epiphytic fitness.

@article{Lindow1993NovelMF,
  title={Novel method for identifying bacterial mutants with reduced epiphytic fitness.},
  author={Steven E. Lindow},
  journal={Applied and environmental microbiology},
  year={1993},
  volume={59 5},
  pages={1586-92}
}
In order to identify novel traits involved in epiphytic colonization, a technique for the rapid identification of bacterial mutants with quantitatively different population sizes in a natural habitat based on measurements of ice nucleation activity was developed. The threshold freezing temperatures of leaves harboring different numbers of cells of ice nucleation-active Pseudomonas syringae B728a differed substantially. While few leaves containing less than about 10 cells per g (fresh weight… CONTINUE READING

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