Novel method for evaluation of the oligomeric structure of membrane proteins.

@article{Ramjeesingh1999NovelMF,
  title={Novel method for evaluation of the oligomeric structure of membrane proteins.},
  author={Mohabir Ramjeesingh and Ling Jun Huan and Elizabeth Garami and Christine E Bear},
  journal={The Biochemical journal},
  year={1999},
  volume={342 ( Pt 1)},
  pages={119-23}
}
Assessment of the quaternary structure of membrane proteins by PAGE has been problematic owing to their relatively poor solubility in non-dissociative detergents. Here we report that several membrane proteins can be readily solubilized in their native quaternary structure with the use of the detergent perfluoro-octanoic acid (PFO). Further, PFO can be used with PAGE, thereby providing a novel, accessible tool with which to assess the molecular mass of homo-multimeric protein complexes. 

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