Novel male-specific molecular markers (MADC5, MADC6) in hemp

@article{Torjek2004NovelMM,
  title={Novel male-specific molecular markers (MADC5, MADC6) in hemp},
  author={Ottó Törjék and N{\'a}ndor Bucherna and Erzs{\'e}bet Kiss and Hajnalka Homoki and Zsuzsanna Finta-Korpelov{\'a} and I. B{\'o}csa and Istv{\'a}n Nagy and L{\'a}szl{\'o} E. Heszky},
  journal={Euphytica},
  year={2004},
  volume={127},
  pages={209-218}
}
Decamer RAPD primers were tested on dioecious and monoecious hemp cultivars to identify sex-specific molecular markers. Two primers (OPD05 and UBC354) generated specific bands in male plants. These two DNA fragments were isolated, cloned and sequenced. Both markers proved to be unique, since no sequence with significant homology to OPD05961 and UBC354151 markers were found in databases. These markers were named MADC3 (OPD05961) and MADC4 (UBC354151) (Male-Associated DNA from Cannabis sativa… 

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References

SHOWING 1-10 OF 36 REFERENCES

Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)

A 400-bp RAPD marker generated by a primer of random decamer sequence has been found associated with the male sex phenotype in 14 dioecious cultivars and accessions of hemp and is suitable for a precise, early and rapid identification of male plants during breeding programs of dIOecious and monoecious hemp.

Application of AFLP for the detection of sex-specific markers in hemp

The abundant number of potential markers for the male sex, their complete cosegregation with male plants and the absence of marker for the female sex support the presence of a male sex chromosome in hemp.

Aflp markers tightly linked to the sex locus in Asparagus officinalis L.

Nine AFLP markers linked to the sex locus in Asparagus officinalis L. have been identified by non-radioactive AFLP technique and bulked segregant analysis and are important to plant breeding, particularly in the development of sex specific PCR primers that could be used in the screening of different asparagus plants at the seedling stage.

Identification of a RAPD marker linked to sex determination in the basket willow (Salix viminalis L.)

A single molecular marker, UBC354560, is biparentally inherited, is associated with femaleness in certain genetic backgrounds, and is linked to allele A1 in the proposed two-locus epistatic genetic model of sex determination for S. viminalis.

Identification of sex in hop (Humulus lupulus) using molecular markers.

Compared pools of male and female plants derived from a hop cross to identify molecular markers associated with the Y or male-specific chromosome, primers developed from the DNA sequence of this product provide a marker for rapid sex identification in crosses of hop by means of PCR.

A pseudoautosomal random amplified polymorphic DNA marker for the sex chromosomes of Silene dioica.

The segregation pattern of an 810-bp random amplified polymorphic DNA (RAPD) band in the F1 and backcross generations of a Silene dioica (L.) Clairv. family provides evidence that this molecular

Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce

Sequence characterized amplified regions (SCARs) were derived from eight random amplified polymorphic DNA (RAPD) markers linked to disease resistance genes in lettuce, providing information on the molecular basis of RAPD markers.

Sex determination in Actinidia. 1. Sex-linked markers and progeny sex ratio in diploid A. chinensis

Results from two strategies aimed at elucidating the genetics of sex in the dioecious genus Actinidia Lindl.

Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis

The OPO08945 RAPD marker could be used in a breeding program to screen the gender of pistachio plants long before they reach reproductive maturity, resulting in considerable savings of time and economic resources.

Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa

The hypothesis that accumulation of a specific LINE-like retrotransposon at the terminal region of the long arm of the Y chromosome might be one cause of heteromorphism of sex chromosomes is supported.