Novel luciferase reporter system for in vitro and organ-specific monitoring of differential gene expression in Listeria monocytogenes.

@article{Bron2006NovelLR,
  title={Novel luciferase reporter system for in vitro and organ-specific monitoring of differential gene expression in Listeria monocytogenes.},
  author={Peter Allard Bron and Ian R Monk and Sin{\'e}ad C. Corr and Colin Hill and Cormac G M Gahan},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 4},
  pages={
          2876-84
        }
}
In this paper we describe construction of a luciferase-based vector, pPL2lux, and use of this vector to study gene expression in Listeria monocytogenes. pPL2lux is a derivative of the listerial integration vector pPL2 and harbors a synthetic luxABCDE operon encoding a fatty acid reductase complex (LuxCDE) involved in synthesis of the fatty aldehyde substrate for the bioluminescence reaction catalyzed by the LuxAB luciferase. We constructed pPL2lux derivatives in which the secA and hlyA… CONTINUE READING

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