Novel listerial genetic loci conferring enhanced barotolerance in Escherichia coli.

@article{Considine2011NovelLG,
  title={Novel listerial genetic loci conferring enhanced barotolerance in Escherichia coli.},
  author={Kiera M Considine and Roy D. Sleator and Alan L Kelly and G. F. Fitzgerald and C. Ray Hill},
  journal={Journal of applied microbiology},
  year={2011},
  volume={110 3},
  pages={618-30}
}
AIMS To identify Listeria monocytogenes genes with a role in high-pressure (HP) resistance. METHODS AND RESULTS A L. monocytogenes genomic library constructed in an Escherichia coli background was screened for loci conferring increased HP resistance. Pressure treatments at 400 megapascals for 5 min in Luria-Bertani (LB) agar were used to screen for increased resistance to pressure. Colonies arising on the treated agar plates were isolated, the plasmid extracted and the inserts sequenced to… CONTINUE READING