Novel insights into the architecture and protein interaction network of yeast eIF3.

@article{Khoshnevis2012NovelII,
  title={Novel insights into the architecture and protein interaction network of yeast eIF3.},
  author={Sohail Khoshnevis and Florian Hauer and Pohl Mil{\'o}n and Holger Stark and Ralf Ficner},
  journal={RNA},
  year={2012},
  volume={18 12},
  pages={2306-19}
}
Translation initiation in eukaryotes is a multistep process requiring the orchestrated interaction of several eukaryotic initiation factors (eIFs). The largest of these factors, eIF3, forms the scaffold for other initiation factors, promoting their binding to the 40S ribosomal subunit. Biochemical and structural studies on eIF3 need highly pure eIF3. However, natively purified eIF3 comprise complexes containing other proteins such as eIF5. Therefore we have established in vitro reconstitution… CONTINUE READING