Novel homologous lactate transporter improves l-lactic acid production from glycerol in recombinant strains of Pichia pastoris

@article{deLima2016NovelHL,
  title={Novel homologous lactate transporter improves l-lactic acid production from glycerol in recombinant strains of Pichia pastoris},
  author={Pollyne Borborema Almeida de Lima and Kelly Cristina Leite Mulder and Nadiele Tamires Moreira Melo and Lucas Silva Carvalho and Gisele Soares Menino and Eduardo Mulinari and Virgilio Hip{\'o}lito Lemos de Castro and Thaila Fernanda dos Reis and Gustavo Henrique Goldman and Beatriz Simas Magalhães and N{\'a}dia Skorupa Parachin},
  journal={Microbial Cell Factories},
  year={2016},
  volume={15}
}
BackgroundCrude glycerol is the main byproduct of the biodiesel industry. Although it can have different applications, its purification is costly. Therefore, in this study a biotechnological route has been proposed for further utilization of crude glycerol in the fermentative production of lactic acid. This acid is largely utilized in food, pharmaceutical, textile, and chemical industries, making it the hydroxycarboxylic acid with the highest market potential worldwide. Currently, industrial… 
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Yeasts for Bioconversion of Crude Glycerol to High-Value Chemicals
Biodiesel production is a fast-growing industry. Biodiesel is obtained through transesterification of different kinds of oils with methanol. This process results in a formation of substantial amounts
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References

SHOWING 1-10 OF 42 REFERENCES
Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli
TLDR
This study demonstrates the efficient conversion of glycerol to L-lactate, a microbial process that had not been reported in the literature prior to this work.
Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum
TLDR
Xylose-assimilating genes encoding xylose isomerase and xylulokinase were cloned into an L-lactate-deficient strain, Lactobacillus plantarum, to produce cost-effective D-lactic acid by using all sugars derived from biomass efficiently.
L-Lactic acid production from glycerol coupled with acetic acid metabolism by Enterococcus faecalis without carbon loss.
TLDR
Gas chromatography-mass spectrometry analysis proved that the E. faecalis strain QU 11 was capable of converting acetic acid to ethanol during lactic acid fermentation of glycerol, and an efficient fermentation system was established without carbon loss.
Recent advances in lactic acid production by microbial fermentation processes.
TLDR
This review will discuss lactic acid producers with relation to their fermentation characteristics and metabolism, and introduces inexpensive fermentative substrates, such as dairy products, food and agro-industrial wastes, glycerol, and algal biomass alternatives to costly pure sugars and food crops.
Genetic Modification of Pichia Pastoris for Production of Propylene Glycol from Glycerol
Biodiesel is emerging as a major renewable energy resource. Glycerol is byproduct of biodiesel production, which cannot be directly used as fuel. Using Pichia pastoris as aerobic yeast cell factory,
Escherichia coli Strains Engineered for Homofermentative Production of d-Lactic Acid from Glycerol
TLDR
The engineered homolactic route for d-lactate production was engineered by overexpressing pathways involved in the conversion of glycerol to this product and blocking those leading to the synthesis of competing by-products, thus representing a viable metabolic pathway.
Modification of metabolic pathways of Saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value
TLDR
The metabolism of yeast is modified by expressing the lactate dehydrogenase (LDH) gene for the production of lactate at low pH values by transforming the recombinant S. cerevisiae strain into a mutant strain lacking the PDC1 gene.
Pichia pastoris fermentation for phytase production using crude glycerol from biodiesel production as the sole carbon source.
Abstract Efficient utilization of crude glycerol, a by-product from biodiesel production, could bring significant economic and environmental benefits. In this work, a low-grade glycerol was used as
L (+)-lactic acid production by pellet-form Rhizopus oryzae NRRL 395 on biodiesel crude glycerol
TLDR
This work introduced sustainable opportunities for L (+)-lactic acid production via R. oryzae NRRL 395 fermentation on biodiesel crude glycerol media, increasing the lactate productivity with the concentration of crude Glycerol.
Efficient Production of l-Lactic Acid by Metabolically Engineered Saccharomyces cerevisiae with a Genome-Integrated l-Lactate Dehydrogenase Gene
TLDR
A metabolically engineered yeast which produces lactic acid efficiently is developed through homologous recombination of the coding region for pyruvate decarboxylase 1 on chromosome XII, and this transgenic strain, which expresses bovine LDH under the control of the PDC1 promoter, also showed high lactic Acid production under nonneutralizing conditions.
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