Novel enzyme immunoassay for thyrotropin‐releasing hormone using N‐(4‐diazophenyl)maleimide as a coupling agent

  title={Novel enzyme immunoassay for thyrotropin‐releasing hormone using N‐(4‐diazophenyl)maleimide as a coupling agent},
  author={Kunio Fujiwara and Tetsuya Saita},
  journal={FEBS Letters},
6 Citations
Méthode de conjugaison d'un polypeptide
La presente invention concerne une methode de conjugaison d'un compose de formule (I) O 5 R1-Q— (ZL-COn-X-N O (I) avec un polypeptide comprenant au moins un groupe thiol, et les molecules obtenues


The preparation and specificity of antibody to thyrotropin releasing hormone.
A double antibody radioimmunoassay for TRH has been developed in which as little as 0.1 ng unlabeled TRH can be detected and a variety of TRH analogues were tested and all had little reactivity in this immunoassays.
Enzyme immunoassay for the quantification of mitomycin C using beta-galactosidase as a label.
Using this assay, serum or urine levels of MMC can be determined accurately after administration of the drug to rats at a single dose of 600 micrograms/kg, and the sensitivity and specificity of the enzyme immunoassay should provide a valuable new tool for use in pharmacokinetic and toxicity studies of M MC.
Thyrotropin-releasing hormone (TRH): distribution in hypothalamic and extrahypothalamic brain tissues of mammalian and submammalian chordates.
Findings indicate that TRH is distributed throughout the vertebrate kingdom, and has an ancient lineage.
Thyrotropin-releasing hormone secretory physiology: studies by radioimmunoassay and affinity chromatography.
These data provide direct documentation that the regulatory control of pituitary TSH secretion by T4 and/or T3 is not mediated by inhibiting the hypothalamic secretion of TRH, but presumably by inhibition of the actions ofTRH at the level of the pituitaries thyrotroph cell.
Development of enzyme immunoassay for chromomycin A3 and olivomycin using beta-D-galactosidase as a label.
A double-antibody enzyme immunoassay for CHM and for the CHM homologue olivomycin was developed which was highly sensitive and accurate enough to measure as little as 10 and 50 pg of each drug per assay tube, respectively.
Distribution and concentration of TRH in the rat brain.
Qualitative and quantitative criteria indicate that TRH is present in methanolic extracts prepared from all regions of the rat brain and the greatest concentration of TRH, determined by radioirnmunoassay, was found in the hypothalamus, followed by the posterior pituitary gland, thalamus, brain stem, cerebrum, anterior pituitaries gland, and cerebellum.