Novel embryo selection techniques to increase embryo implantation in IVF attempts

  title={Novel embryo selection techniques to increase embryo implantation in IVF attempts},
  author={George Α. Sigalos and Olga Triantafyllidou and Nikos F Vlahos},
  journal={Archives of Gynecology and Obstetrics},
PurposeThe final success of an IVF attempt depends on several steps and decisions taken during the ovarian stimulation, the oocyte retrieval, the embryo culture and the embryo transfer. The final selection of the embryos most likely to implant is the final step in this process and the responsibility of the lab. Apart from strict morphologic criteria that historically have been used in embryo selection, additional information on genetic, metabolomic and morphokinetic characteristics of the… Expand
Euploid embryos selected by an automated time-lapse system have superior SET outcomes than selected solely by conventional morphology assessment
Automated TLI combined with PGS is a useful prognostic tool to identify euploid embryos with the highest potential for implantation and pregnancy, and results provide evidence that a healthy pregnancy does not only depend upon normal chromosomal status. Expand
Day 3 embryo transfer versus day 5 blastocyst transfers: A prospective randomized controlled trial
The efficacy of blastocyst transfer is not inferior to that of embryo transfer on cleavage stage and may have benefits because it is associated with acceptable pregnancy rates and morphologic assessment on day 3 has limited predictive value for subsequent embryonic development. Expand
Analysis of the Relationship between CGB5 155G/C Polymorphism and in vitro Fertilization-embryo Transfer Outcome (IVF-ET) in the Iranian Population
The results of this study suggested that CGB5 (-155G/C) CC genotype has a protective effect on IVF-ET outcome. Expand
Pronuclear and blastocyst morphology are associated age-dependently with embryo ploidy in in vitro fertilization cycles.
The data showed that pronuclear and blastocyst morphology are associated withblastocyst ploidy in younger patients, which may help embryo selection for embryo transfer and decision-making on which blastocysts should be biopsied in PGT-A cycles. Expand
The effect on twinning rate of transferring double vitrified-warmed embryos in women of advanced reproductive age: a retrospective study
It was concluded that women with a favorable or average prognosis have a high risk of twin pregnancies and this finding can be used to guide future practice: that is, performing eSET in women with favorable oraverage prognosis and DET in womenwith poor prognosis. Expand
Dysregulation of the interleukin-17A pathway in endometrial tissue from women with unexplained infertility affects pregnancy outcome following assisted reproductive treatment.
Dysregulated interleukin-17 pathway components are demonstrated in women who fail to become pregnant after ART, and elevated serum levels of the pro-inflammatory cytokine IL-17 may predict failure of ART in women with unexplained infertility. Expand
Increased copy number of syncytin-1 in the trophectoderm is associated with implantation of the blastocyst
The increased copy number of syncytin-1 in the blastocyst trophectoderm was associated with good outcomes of pregnancies and was significantly higher in the pregnant group than in the non-pregnant group. Expand
The impact of accurately timed mid-luteal endometrial injury in nulligravid women undergoing their first or second embryo transfer
No differences in rates of implantation, clinical pregnancy or live birth in women with primary infertility were determined between those who underwent a scratch and those who did not. Expand


New approaches to embryo selection.
The most promising strategy at present is the combined evaluation of morphology and developmental kinetics using time-lapse imaging, which brought new insights into certain characteristics that enable deselection of embryos at an early stage of development and to identify others with high potential for successful implantation. Expand
Cleavage stage versus blastocyst stage embryo transfer in assisted conception.
If blastocyst stage embryo transfers (ETs) affects success rates compared with cleavage stage ETs and investigate what factors may influence this is investigated. Expand
Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology.
There is evidence that there is a small significant difference in live birth rates in favour of blastocyst transfer ( Day 5 to 6) compared to cleavage stage transfer (Day 2 to 3). Expand
Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover.
Non-invasive assay of amino acid turnover has the potential to improve significantly the prospective selection of the most viable embryos, or single embryo, for replacement in an IVF cycle. Expand
The use of morphokinetics as a predictor of embryo implantation.
A multivariable model is proposed based on the findings to classify embryos according to their probability of implantation and it is proposed that the image acquisition and time-lapse analysis system makes it possible to determine exact timing of embryo cleavages in a clinical setting. Expand
Morphological and cytogenetic assessment of cleavage and blastocyst stage embryos.
It is confirmed that, at the cleavage stage, chromosome abnormalities have little if any effect on morphological scores assigned using traditional criteria, and in the case of the most clinically relevant aneuploidies there was no detectable effect on morphology at any preimplantation stage. Expand
Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial.
Cleavage-stage biopsy markedly reduced embryonic reproductive potential and in contrast, trophectoderm biopsy had no measurable impact and may be used safely when embryo biopsy is indicated. Expand
Time-lapse systems for embryo incubation and assessment in assisted reproduction.
There is no evidence of a difference between the interventions in terms of live birth rates, and the main limitations were imprecision and risk of bias associated with lack of blinding of participants and researchers, and indirectness secondary to significant heterogeneity between interventions in some studies. Expand
Clinical application of comprehensive chromosomal screening at the blastocyst stage.
The comprehensive chromosome screening method described overcomes many of the problems that limited earlier aneuploidy screening techniques and may finally allow preimplantation genetic screening to achieve the benefits predicted by theory. Expand
Reply: Clinical outcomes following selection of human preimplantation embryos with time-lapse monitoring: a systematic review.
While TLM has the potential to revolutionize clinical embryology, there are currently no high-quality data to support the clinical use of this technology for selection of human preimplantation embryos and recommendations for the adoption are limited by the available literature and the lack of robust prospective studies reporting clinical outcomes. Expand