Novel droplet vitrification combined with fish antifreeze protein type III enhances cryoprotection of semen in wild endangered Persian sturgeon Acipenser persicus (Borodin, 1897)

@inproceedings{AbedElmdoust2015NovelDV,
  title={Novel droplet vitrification combined with fish antifreeze protein type III enhances cryoprotection of semen in wild endangered Persian sturgeon Acipenser persicus (Borodin, 1897)},
  author={Amirreza Abed-Elmdoust and Hamid Farahmand and Bagher Mojazi-Amiri and Gholamreza Rafiee and Ruhollah Rahimi},
  year={2015}
}
AbstractIn this study, the efficiency of a novel dropletvitrification technique along with different dosesof fish antifreeze protein (AFP) type III on Per-sian sturgeon thawed spermatozoa quality (motil-ity duration and motility percentage) wasinvestigated. Semen of seven male individualswas pooled in equal volumes and diluted with4°C Tris-Hcl (100 mM), pH = 8 extenders con-taining 0, 5, 10, 15 lM of AFP type III in aratio of 1:1 (semen/extenders). Treated semenwas dropped into liquid nitrogen… CONTINUE READING

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