Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency

@inproceedings{Diecke2015NovelCM,
  title={Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency},
  author={Sebastian Diecke and Jiamiao Lu and Jaecheol Lee and Vittavat Termglinchan and Nigel G. Kooreman and Paul W. Burridge and Antje D. Ebert and Jared M. Churko and Arun Sharma and Mark A. Kay and Joseph C. Wu},
  booktitle={Scientific reports},
  year={2015}
}
The development of human induced pluripotent stem cell (iPSC) technology has revolutionized the regenerative medicine field. This technology provides a powerful tool for disease modeling and drug screening approaches. To circumvent the risk of random integration into the host genome caused by retroviruses, non-integrating reprogramming methods have been developed. However, these techniques are relatively inefficient or expensive. The mini-intronic plasmid (MIP) is an alternative, robust… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 21 CITATIONS

Pathological ASXL1 Mutations and Protein Variants Impair Neural Crest Development

VIEW 2 EXCERPTS
CITES METHODS

Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo.

VIEW 2 EXCERPTS
CITES METHODS

Modeling Short QT Syndrome Using Human‐Induced Pluripotent Stem Cell–Derived Cardiomyocytes

VIEW 1 EXCERPT
CITES METHODS

References

Publications referenced by this paper.
SHOWING 1-10 OF 51 REFERENCES

Generation of induced pluripotent stem cells.

the technologies of iPSC generation

  • Hu, K. All roads lead to induced pluripotent stem cells
  • Stem cells dev 23, 1285–1300
  • 2014

A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro.

Efficient generation of human iPSCs by a synthetic selfreplicative RNA

  • N Yoshioka
  • Cell Stem Cell 13,
  • 2013