Novel characteristics and regulation of a divergent cinnamate 4-hydroxylase (CYP73A15) from French bean: engineering expression in yeast

@article{Nedelkina1999NovelCA,
  title={Novel characteristics and regulation of a divergent cinnamate 4-hydroxylase (CYP73A15) from French bean: engineering expression in yeast},
  author={Svetlana Nedelkina and Stephen C. Jupe and Kristopher A. Blee and Michel Schalk and Daniele Werck-reichhart and Gregory Bolwell},
  journal={Plant Molecular Biology},
  year={1999},
  volume={39},
  pages={1079-1090}
}
cDNAs showing high sequence similarity (>70%) over large stretches to plant CYP73A orthologues from other species were isolated from a cDNA library derived from mRNAs expressed in elicitor-treated suspension-cultured cells. These clones appear to code for a full-length 1554 bp open reading frame with a 78 bp 5′-untranslated region and a 140 bp 3′-untranslated region. The open reading frame, determined by sequence similarity, codes for a protein with a predicted Mr of 59 229 and a pI of 8.8. It… CONTINUE READING
16 Citations
49 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 49 references

Molecular cloning and sequencing of a cDNA encoding mung bean cytochrome P450 (P450C4H) possessing cinnamate 4-hydroxylase activity

  • M Mitzutani, E Ward, J DiMaio, D Ohta, J Ryals, R Sato
  • Biochem Biophys Res Comm 190: 875–880
  • 1993
Highly Influential
4 Excerpts

Catalytic reactivities and structure/function relationships of cytochrome P450 enzymes

  • B Halkier
  • Phytochemistry 43: 1–21
  • 1996

Splicing of precursors to mRNA in higher plants: mechanisms, regulation and subnuclear organization of the spliceosomal machinery

  • GG Simpson, W Filipowics
  • Plant Mol Biol 32: 1–41
  • 1996
1 Excerpt

Similar Papers

Loading similar papers…