Novel bifunctional probe for radioisotope-free photoaffinity labeling: compact structure comprised of photospecific ligand ligation and detectable tag anchoring units.

@article{Hosoya2004NovelBP,
  title={Novel bifunctional probe for radioisotope-free photoaffinity labeling: compact structure comprised of photospecific ligand ligation and detectable tag anchoring units.},
  author={Takamitsu Hosoya and Toshiyuki Hiramatsu and Takaaki Ikemoto and Masayuki Nakanishi and Hiroshi Aoyama and Ayako Hosoya and Tomoya Iwata and Kei Maruyama and Makoto Endo and Masaaki Suzuki},
  journal={Organic & biomolecular chemistry},
  year={2004},
  volume={2 5},
  pages={637-41}
}
A novel method for radioisotope-free photoaffinity labeling was developed, in which a bifunctional ligand is connected to a target protein by activation of a photoreactive group, such as an aromatic azido or 3-trifluoromethyl-3H-diazirin-3-yl group, and identification of the ligated product is achieved by anchoring of a detectable tag through the Staudinger-Bertozzi reaction with an alkyl azido moiety that survives photolysis. The chemical ground of this method was confirmed using model… CONTINUE READING