Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3.

@article{Ishikawa2001NovelBH,
  title={Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3.},
  author={Kazuhiko Ishikawa and Hide-ki Ishida and Ikuo Matsui and Yutaka Kawarabayasi and Hisasi Kikuchi},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 2},
  pages={673-9}
}
Genome sequencing of the thermophilic archaeon Pyrococcus horikoshii OT3 revealed a gene which had high sequence similarity to the gene encoding the carboxypeptidase of Sulfolobus solfataricus and also to that encoding the aminoacylase from Bacillus stearothermophilus. The gene from P. horikoshii comprises an open reading frame of 1,164 bp with an ATG initiation codon and a TGA termination codon, encoding a 43,058-Da protein of 387 amino acid residues. However, some of the proposed active-site… CONTINUE READING

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