Novel aspects of tomato root colonization and infection by Fusarium oxysporum f. sp. radicis-lycopersici revealed by confocal laser scanning microscopic analysis using the green fluorescent protein as a marker.

@article{Lagopodi2002NovelAO,
  title={Novel aspects of tomato root colonization and infection by Fusarium oxysporum f. sp. radicis-lycopersici revealed by confocal laser scanning microscopic analysis using the green fluorescent protein as a marker.},
  author={Anastasia L. Lagopodi and Arthur F. J. Ram and Gerda Lamers and Peter J. Punt and Cees A. M. J. J. van den Hondel and Ben Lugtenberg and Guido Vincent Bloemberg},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2002},
  volume={15 2},
  pages={172-9}
}
The fungus Fusarium oxysporum f. sp. radicis-lycopersici is the causal agent of tomato foot and root rot disease. The green fluorescent protein (GFP) was used to mark this fungus in order to visualize and analyze the colonization and infection processes in vivo. Transformation of F oxysporum f. sp. radicis-lycopersici was very efficient and gfp expression was stable for at least nine subcultures. Microscopic analysis of the transformants revealed homogeneity of the fluorescent signal, which was… CONTINUE READING
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