Novel approach for developing urinary nucleosides profile by capillary electrophoresis-mass spectrometry.

@article{Wang2007NovelAF,
  title={Novel approach for developing urinary nucleosides profile by capillary electrophoresis-mass spectrometry.},
  author={Shufang Wang and Xiaoping Zhao and Yong Mao and Yiyu Cheng},
  journal={Journal of chromatography. A},
  year={2007},
  volume={1147 2},
  pages={254-60}
}
A simple, rapid and efficient capillary electrophoresis-mass spectrometry (CE-MS) method was developed to analyze urinary nucleosides for the first time. The composition of CE buffer and MS parameters were systematically optimized. The optimum buffer was 150 mM acetic acid containing 15% methanol and 15% ethanol. The optimum MS parameters were: methanol containing 0.5% acetic acid was selected as the sheath liquid and the flow rate was 5 microL/min; the flow rate and temperature of drying gas… CONTINUE READING