Novel approach for accurate tissue-based protein colocalization and proximity microscopy

@inproceedings{Lutz2017NovelAF,
  title={Novel approach for accurate tissue-based protein colocalization and proximity microscopy},
  author={Mirjam I. Lutz and Carmen Schwaiger and Bernhard Hochreiter and Gabor G Kovacs and Johannes A. Schmid},
  booktitle={Scientific Reports},
  year={2017}
}
Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could… CONTINUE READING
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