Novel application of reversed-phase UPLC-oaTOF-MS for lipid analysis in complex biological mixtures: a new tool for lipidomics.

@article{Rainville2007NovelAO,
  title={Novel application of reversed-phase UPLC-oaTOF-MS for lipid analysis in complex biological mixtures: a new tool for lipidomics.},
  author={Paul D. Rainville and Chris L Stumpf and John P. Shockcor and Robert S. Plumb and Jeremy K. Nicholson},
  journal={Journal of proteome research},
  year={2007},
  volume={6 2},
  pages={552-8}
}
Ultra-Performance LC (UPLC) utilizing sub-2-mum porous stationary phase particles operating with high linear velocities at pressures >9000 psi was coupled with orthogonal acceleration time-of-flight (oaTOF) mass spectrometry and successfully employed for the rapid separation of lipids from complex matrices. The UPLC system produced information-rich chromatograms with typical measured peak widths of 3 s at peak base, generating peak capacities in excess of 200 in 10 min. Further UPLC coupled… CONTINUE READING