Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies.

@article{Hanekom2004NovelAO,
  title={Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies.},
  author={Willem Albert Hanekom and Jane Hughes and Maushumi P Mavinkurve and Megan Mendillo and Marcia L V Watkins and Hoyam Gamieldien and Sebastian J. Gelderbloem and Mzwandile Sidibana and Nazma Mansoor and Virginia Davids and Rose Ann Murray and Anthony Hawkridge and Patrick A. J. Haslett and Stanley R Ress and Gregory Dudley Hussey and Gilla Kaplan},
  journal={Journal of immunological methods},
  year={2004},
  volume={291 1-2},
  pages={185-95}
}
We optimized a whole blood intracellular cytokine assay to quantitate the frequency of specific CD4+ and CD8+ T cells in small volumes of whole blood from infants from developing countries. The assay is performed in two steps. First, whole blood is stimulated in the presence of specific antigens for 6-18 h, ending with cryopreservation of fixed white cells. These stimulation steps were specifically adapted to be practical and reliable in a rural, developing country field setting. Later, in a… CONTINUE READING

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