• Corpus ID: 36586302

Novel anti-Tn single-chain Fv-Fc fusion proteins derived from immunized phage library and antibody Fc domain.

@article{Kubota2010NovelAS,
  title={Novel anti-Tn single-chain Fv-Fc fusion proteins derived from immunized phage library and antibody Fc domain.},
  author={Tsuguo Kubota and Takefumi Matsushita and Rinpei Niwa and Izumi Kumagai and Kazuyasu Machida-shi Nakamura},
  journal={Anticancer research},
  year={2010},
  volume={30 9},
  pages={
          3397-405
        }
}
Tn[GalNAc(α1-3)-Ser/Thr] antigen, a tumor-associated carbohydrate antigen, is highly expressed in various tumors and an attractive candidate for cancer immunotherapy. The generation of an anti-Tn antibody is a first step toward the construction of new anticancer molecules. However, because of the simple and small conformation of the Tn molecule, it is difficult to generate an anti-Tn antibody for therapeutic use by conventional hybridoma technology. The purpose of this study was to isolate anti… 

Figures and Tables from this paper

Renewed interest in basic and applied research involving monoclonal antibodies against an oncofetal Tn-antigen.
TLDR
The recent studies demonstrated that MLS128 significantly inhibits breast and colon cancer cell growth and Thermodynamic studies on MLS128 binding to Tn2/Tn3 revealed its unique nature of temperature-dependent binding.
Challenges in Antibody Development against Tn and Sialyl-Tn Antigens
TLDR
The development of novel antibodies against Tn and STn taking advantage of innovative technologies and engineering techniques may result in innovative therapeutic antibodies for cancer treatment.
Identification of Tn Antigen O-GalNAc-expressing glycoproteins in human carcinomas using novel anti-Tn recombinant antibodies.
TLDR
Rec recombinant Remab6 and ReBaGs6 are useful for biochemical characterization of cancer cells and IHC of tumors, and represent promising tools for Tn biomarker discovery independently of recognition of IgA1.
Peptide-displaying phage technology in glycobiology.
TLDR
This review provides examples of successful applications of peptide-displaying phage technology to glycobiology and suggests an approach should benefit translational research by supplying carbohydrate-mimetic peptides and carbohydrate-binding polypeptides.
Generation of a Fully Human scFv that binds Tumor-Specific Glycoforms
TLDR
Validation studies in vitro and in vivo using scFvC9 indicate this antibody can be useful for the development of diagnostic, imaging, and therapeutic applications for cancers that express the antigen.
The Cosmc connection to the Tn antigen in cancer.
TLDR
Exposure to the Tn antigen by disruption of either C1GalT (T-syn) or Cosmc in mice is associated with a tremendous range of pathologies and engineered expression of theTn antigen in mouse embryos leads to embryonic death.
Tn and sialyl‐Tn antigens, aberrant O‐glycomics as human disease markers
TLDR
Both Tn and STn are tumor‐associated carbohydrate antigens and tumor biomarkers, since they are not expressed normally and appear early in tumorigenesis, and their expression is strongly associated with poor prognosis and tumor metastasis.
A new technology for increasing therapeutic protein levels in the brain over extended periods
TLDR
A new technology is reported, named AccumuBrain, that achieves both high antibody concentration in the brain and a long half-life in blood by binding to myelin oligodendrocyte glycoprotein (MOG), which is specifically expressed in oligodendedrocytes.
...
1
2
...

References

SHOWING 1-10 OF 42 REFERENCES
Phage-display selection of a human single-chain fv antibody highly specific for melanoma and breast cancer cells using a chemoenzymatically synthesized G(M3)-carbohydrate antigen.
TLDR
It is demonstrated that a synthetic G( M3) panning reagent can be used to isolate a fully human scFv that is highly specific for native G(M3) on the surface of tumor cells.
Expression of single-chain Fv-Fc fusions in Pichia pastoris.
Anti-Tumor Immunity Provided by a Synthetic Multiple Antigenic Glycopeptide Displaying a Tri-Tn Glycotope1
TLDR
Both the clustering of carbohydrate Ags and the way they are displayed seem to be important parameters for stimulating efficient anti-saccharide immune responses.
Selection of large diversities of antiidiotypic antibody fragments by phage display.
TLDR
A fast and reliable technique for generating large diversities of antiidiotypic single chain antibody fragments from non-immunized phagemid libraries using phage display is described, enabling for the first time the generation of a large variety of Ab2s after only two to three rounds of selection, thereby maintaining maximum diversity.
A novel approach for immunization, screening and characterization of selected scFv libraries using membrane fractions of tumor cells.
TLDR
A rapid and more versatile method for isolating antibody fragments compared to whole cell screening procedures is described and can be stored for at least 15 months at -80 degrees C and used to immunize mice or for screening of antibody libraries.
Secretion of a single-gene-encoded immunoglobulin from myeloma cells.
TLDR
The single-gene construct approach provides a way of generating an immunoglobulin-like molecule which retains the specificity, binding properties, and cytolytic activity of the chimeric mAb CC49, potentially a therapeutic and diagnostic reagent against a range of human carcinomas.
...
1
2
3
4
5
...